Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Evaluation of differentiation potential of SF-MSCs and bone marrow (BM)-MSCs by gene expressions. Gene expression profiles of SF-MSCs and BM-MSCs after osteogenic, chondrogenic, and adipogenic induction. Among them, type I collagen, osteocalcin, and osteopontin were for osteogenesis; type II collagen and aggrecan were for chondrogenesis; and peroxisome proliferator activated receptor γ 2 (PPAγ2) and adipocyte protein 2 (aP2) were for adipogenesis, respectively. Three SF-MSCs and BM-MSCs samples were performed for this experiment.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Evaluation of differentiation potential of SF-MSCs by histological staining and immunofluorescence staining. Histological staining ( A ) and immunofluorescence staining ( B ) of SF-MSCs after osteogenic (Alizarin red S stain and Type I collagen), chondrogenic (Alcain blue stain and Type II collagen), and adipogenic (Oil red O stain and PPAγ2) induction. Green: extracellular matrix and blue: cell nuclei.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: Staining, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Sequences of primers used in real-time PCR.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques:

Journal: PLoS ONE

Article Title: Bruton's tyrosine kinase inhibitor BMS-986142 in experimental models of rheumatoid arthritis enhances efficacy of agents representing clinical standard-of-care

doi: 10.1371/journal.pone.0181782

Figure Lengend Snippet: ( A ) Mean clinical scores over the course of the study, ( B ) mean clinical scores at the end of the study (day 46), ( C ) histological evaluation of the right hind paws, (D) plasma cells as measured by FACS analysis performed on spleens from 5 mice per group (3 mice in naïve group; non-immunized mice), (E) CD38 expression (MFI) on splenic CD138+B220low plasma cells, (F) anti-collagen II IgG titers, and ( G ) pharmacokinetics of BMS-986142 measured on the last day of the study with the data represented as time after the morning dose. The dashed line represents the IC50 value determined in vitro against BCR-stimulated CD69 expression on B cells in mouse whole blood. Data for B through F shown as mean ± SEM. * p < 0.05 versus vehicle group, # p < 0.05 versus either treatment alone, n = 10/group.

Article Snippet: A mixture of 4 monoclonal anti-mouse type II collagen antibodies (1 mg of each) was administered IP to female BALB/c mice (Harlan Laboratories; 8–10 weeks old) [ ].

Techniques: Clinical Proteomics, Expressing, Drug discovery, In Vitro